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. 2020 Oct 20;16(10):e1008945. doi: 10.1371/journal.ppat.1008945

Fig 9. L-FMAU-arrested HBV RNA-virions are non-infectious.

Fig 9

HepG2-NTCP12 cells in 96-well plates were infected with the wild-type HBV virions (DNA virions) and the RNA virions collected from L-FMAU-treated HepAD38 cells, respectively, at MOI of 500 vge/cell. Uninfected cells served as mock control. 10 days later, the infection was assessed by (A) intracellular HBcAg immunofluorescence, (B) supernatant HBeAg ELISA, and (C) HBV cccDNA qPCR. DAPI staining of cell nuclei served as counter staining. The intracellular cccDNA copy numbers were normalized by mitochondrial DNA and plotted as relative levels of control (fold change of 1) (mean±SD, n = 3).