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. 2020 Sep 24;1:111–115. doi: 10.1016/j.crtox.2020.09.003

Fig. 2.

Fig. 2

Effects of KIF and AUY-922 on P53 regulation.

Western Bot analysis of (A) P53 and β-actin, (B) MDM2 and β-actin expression after 24 h treatment of BPAEC with either KIF (5 μM) or vehicle (0.1% DMSO); and post-treatment with AUY-922 (2 μM) or vehicle (0.1% DMSO) for 16 h. The blots shown are representative of 3 independent experiments. The signal intensity of P53 and MDM2 was analyzed by densitometry, and the protein levels were normalized to β-actin. *P < 0.05, **P < 0.01 vs vehicle. Means ± SEM. Western Bot analysis of (C) P53 and β-actin, (D) MDM2 and β-actin expression after 24 h treatment of HuLEC-5a with either KIF (5 μM) or vehicle (0.1% DMSO) and post-treatment with AUY-922 (2 μM) or vehicle (0.1% DMSO) for 16 h. The blots shown are representative of 3 independent experiments. Signal intensity of P53 and MDM2 was analyzed by densitometry. Protein levels were normalized to β-actin. *P < 0.05, *** < 0.001 vs vehicle. Means ± SEM.