Figure 4.
UCC1 and UCC2 Are Necessary for the Central Lignification of the Casparian Strip
(A) Surface view of the Casparian strip lignin stained with Basic fuchsin in WT plants and the mutants ucc2.1, ucc1.1, ucc1.2, and ucc1.2 ucc2.1. Whites arrows show lack of lignification in the central domain of the Casparian strip across the different genotypes. Scale bar, 2 μm. see also Figure S4A.
(B) Quantification of normalized pixels intensity (0–1) (relative pixel intensity) across the Casparian strip using surface views as shown in (A). The plots show the intensity profile for individual plants (n ≥ 13). In the figure, the mean value is represented by a black line and the 95% confidence interval is in gray. The data were generated using individual pictures containing a Casparian strip segment of approximately 25 μm long. The pictures were taken at the 15th cells after the onset of elongation and the intensity profiles were measured in the same direction, from the cortex side toward the pericycle side of the endodermis. Letters indicate statistically significant differences between genotypes using an ANOVA and Tukey’s test as post hoc analysis (p < 0.01) (see also Figure S4B).