REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-UCC1	This paper	N/A
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 633	Invitrogen	SA5-10034, RRID:AB_2556614
Chemicals, Peptides, and Recombinant Proteins
MS basal salt mixture, powder	Sigma-Aldrich	M5524
Agar	Sigma-Aldrich	P49805, CAS: 94-53A7921, CASS: 9002-18-01
Piperonylic acid	Sigma-Aldrich	P49805, CAS: 94-53-1
guanidine-HCl	Sigma-Aldrich	G7294
MES	Sigma-Aldrich	M8250 CAS: 1266615-59-1
EDTA	Sigma-Aldrich	EDS, CAS:60-00-4
Phenol:chloroform:isoamylalcohol, 25:24:1	Sigma-Aldrich	P2069
Acetic acid	Sigma-Aldrich	A6283 CAS: 64-19-7
Sodium acetate	Sigma-Aldrich	S2889 CAS, 127-09-3
DNase I, Amplification Grade	Invitrogen	18068015
Nitric acid Primar Plus	Fisher Chemicals	N/2272/PB17 CAS: 7697-37-2
Propidium iodide	Invitrogen	P1304MP, CAS: 25535-16-4
Calcofluor White	Polysciences,	CAT#4359
Basic Fuchsin	Fluka, Analytical	CAS: 58969-01-0
Auramine-O	Sigma-Aldrich	SLA 1027 CAS: 2465-27-2
paraformaldehyde	Sigma-Aldrich	P6148, CAS:3052-89-4
xylitol	Sigma-Aldrich	W507930, CAS: 87-99-0
Sodium deoxycholate	Sigma-Aldrich	D6750, CAS: 302-95-4
urea	Sigma-Aldrich	U5378, CAS:57-13-6
Fluorol Yellow 088	Santa Cruz Biotechnology	SC215052, CAS: 81-37-8
PIPES	Sigma-Aldrich	P-3768, CAS: 76836-02-7
EGTA	Sigma-Aldrich	E3889, CAS: 67-42-5
MgSO4·7H2O	Sigma-Aldrich	63138, CAS:10034-99-8
KOH	Sigma-Aldrich	P5958, CAS:1310-58-3
Methanol	Sigma-Aldrich	82762, CAS:67-56-1
Driselase	Sigma-Aldrich	D9515, CAS:85186-71-6
Macerozyme	Duchefa	cat. N M8002.0010
IGEPAL CA-630	Sigma-Aldrich	I8896, CAS:9002-93-1
DMSO	Sigma-Aldrich	D8418, CAS: 67-68-5
albumin fraction V BSA	Sigma-Aldrich	10735078001
Critical Commercial Assays
Qubit	Invitrogen	Q10210
TapeStation	Aglent	G2991A
Lexogen Quant Seq 3′ mRNA Seq (FWD) Library Prep Kit	Lexogen	015
Experimental Models: Organisms/Strains
Arabidopsis thaliana Columbia 0 accession (Col-0)
ucc1.1, CRISPR line in Col-0	This study	N/A
ucc1.2, CRISPR line in Col-0	This study	N/A
sgn3.3, t-DNA line in Col-0	[5]	SALK_043282
myb36.2, t-DNA line in Col-0	[2]	GK-543B11
casp1.1 casp3.1, t-DNA line in Col-0	[7]	N/A
pCASP1::CASP1-GFP, line in Col-0	[7]	N/A
pESB1::ESB1-mCherry, line in Col-0	[6]	N/A
ucc2.1, t-DNA line in Col-0	N/A	GK_250F04
ucc2.2, t-DNA line in Col-0	N/A	salk_049638
Rbohf, t-DNA line in Col-0	[15]	salk_059888
pUCC1::mCherry-UCC1, line in Col-0	This study	N/A
pUCC1::GFP-GUS, line in Col-0	This study	N/A
pUCC1::UCC1, line in Col-0	This study	N/A
Oligonucleotides
For pUCC1::mCherry-UCC1 GGGGACAACTTTGTATAGAAAAGTTG GTTGAATTTCGTAAGAGTTAGG	This study	N/A
For pUCC1::mCherry-UCC1 GGGGACTGCTTTTTTGTACAAACTTG CATGGTCAGTAGCTACTGTTAAACC	This study	N/A
For pUCC1::mCherry-UCC1 GGGGACAGCTTTCTTGTACAAAGTG GTAACCATTGGTGGTCCTAGTGGTTGG	This study	N/A
For pUCC1::mCherry-UCC1 GGGGACAACTTTGTATAATAAAGTTG ACCCATATAAATTGTAATAATGTATTATAAAC	This study	N/A
For pUCC1::UCC1 GGGGACAACTTTGTATAGAAAAGTTG GTTGAATTTCGTAAGAGTTAGG	This study	N/A
For pUCC1::UCC1 GGGGACTGCTTTTTTGTACAAACTTGG ATGACATATGGTGTCAAATGTGTG	This study	N/A
For pUCC1::UCC1 GACAAGTTTGTACAAAAAAGCAGGCTCG GCACAAAACATCATCATCTCTTG	This study	N/A
For pUCC1::UCC1 GGGGACCACTTTGTACAAGAAAGCTGGGT CCCATATAAATTGTAATAATGTATTATAAAC	This study	N/A
For pUCC1::GFP-GUS GGGGACAAGTTTGTACAAAAAAGCAGGCT GTTGAATTTCGTAAGAGTTAGG	This study	N/A
For pUCC1::GFP-GUS GGGGACCACTTTGTACAAGAAAGCTGGGT ATGACATATGGTGTCAAATGTGTG	This study	N/A
Recombinant DNA
pUCC1::mCherry-UCC1	This study	N/A
pUCC1::GFP-GUS	This study	N/A
pEN-L1-mCherry-L2	VIB [34],	https://gatewayvectors.vib.be/collection/pen-l1-mcherry-l2
pB7m34GW,3	VIB [34],	https://gatewayvectors.vib.be/collection/pb7m34gw0
pH7m24GW	VIB [34],	https://gatewayvectors.vib.be/collection/ph7m24gw2
pBGWFS7	VIB [35],	https://gatewayvectors.vib.be/collection/pbgwfs7
pUCC1::UCC1	This study	N/A
pHEE401	[36]	Addgene Plasmid # 71286
Software and Algorithms
Fiji	[37]	N/A
R	The R Project for Statistical Computing	N/A
PlotTwist	[38]	N/A
RStudio	RStudio	N/A