Figure 2.
Oryzalin Treatment and Cell Division in Developing Leaves and Sepals
(A–F) Arabidopsis sepal development after the treatment of DMSO (A–C) or oryzalin (D–F) for 0 h (A and D) and 48 h (B, C, E, and F). Note that the growth continues after the oryzalin treatment. Cross sections of sepals in (B) and (E) are shown in (C) and (F).
(G) Quantification of width/thickness ratios. Treated sepals do not flatten (n = 3 biological repeats).
(H and I) The morphology of tomato P2 48 h after the treatment with DMSO (H) or oryzalin (I). Arrows indicate the primordia treated with chemicals. Leaf primordium treated with DMSO show normal anisotropic growth and generate lateral leaflet primordia (asterisks). The anisotropic growth and planar leaf form are compromised in oryzalin treated samples.
(J) Cell division pattern by mPS-PI staining in optical cross sections of tomato P2 treated with DMSO (left column) or oryzalin (right column) for 24 h. White, divisions perpendicular to the epidermis; blue, divisions parallel (angle < 30°) to mediolateral axis in inner cells or to the epidermis; green, other divisions (30° < angle < 90°).
(K) Quantification of cell division pattern in (J).
For DMSO treatment, n = 146 cells, and for oryzalin treatment, n = 91 cells. Scale bars, 20 μm in (A)–(F) and (J) 100 μm (H) and (I).