FIGURE 4:

β1-integrin knockout promotes astrocyte differentiation. Female control or β1 KO tissue was analyzed at 14 dpi. A: Immunostaining for YFP (green) and GFAP (red) shows that β1-integrin knockout increases colocalization of YFP⁺ recombined cells with the astrocyte marker GFAP. B: Immunostaining for YFP (green) and ALDH1L1 (red) shows that β1-integrin knockout increases colocalization of YFP⁺recombined cells with the astrocyte marker ALDH1L1. C: Immunostaining for YFP (green) and S100β (red) shows that β1-integrin knockout increases colocalization of YFP⁺ recombined cells with the astrocyte marker S100β. D: Quantification of GFAP⁺YFP⁺ cells, ALDH1L1⁺YFP⁺ cells, and S100β⁺YFP⁺ cells shows that β1-integrin knockout increases astrocyte differentiation. Scale bar 50 µm. Data are means ± SEM. Unpaired Student’s t test: *P < 0.05. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]