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. 2020 Oct 20;6:106. doi: 10.1038/s41420-020-00340-9

Fig. 1. Generation and characterization of cardiac-specific HMGB1 knockout mice.

Fig. 1

a Schematic diagram of the mouse breeding strategy to generate cardiac-specific HMGB1 knockout mice. b The mRNA levels of the Hmgb1 gene in control and HMGB1△CM hearts measured by quantitative RT-PCR at neonatal and 12-week-old. n = 4 mice per group. *p < 0.05 versus control, t-test, mean ± SEM. c Western blot analysis of HMGB1 protein in the whole-heart tissue of control and HMGB1△CM hearts at neonatal and 12-week old. ACTB was used as the loading control for the whole heart. n = 4 mice per group. d Immunostaining of the heart at the neonatal stage, bar = 25 μm. e Immunostaining of the hearts of HMGB1 mice at 12-week-old, bar = 100 μm.