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. 2020 Oct 20;10:17784. doi: 10.1038/s41598-020-74460-8

Table 1.

Key ingredients and macronutrient composition of the commercially available rodent diets.

Rodent diet tested Key ingredients (in order of weight) Protein % kcal Fat % kcal Carbohydrate % kcal Fibre (crude) % kcal/g
Purified ingredient diets
Research Diets, AIN93G Growing Rodent purified ingredient diet Per 1000 g: 397.49 g Corn starch, 200 g casein, 132 g Lodex 10, 100 g sucrose, 70 g soybean oil, 50 g Solka Floc FCC200, 35 g mineral S10022G, 10 g vitamin V10037, 3 g cystine, 2.5 g choline bitartrate, 0.01 g tert-butylhydroquinone 21 12 67 5.0 3.86
Research Diets, RD Western purified ingredient diet Per 1000 g : 350 g Sucrose, 200 g anhydrous butter, 195 g casein, 100 g Lodex 10, 50 g corn starch, 50 g Solka Floc FCC200, 17.5 g mineral S10001A, 17.5 g Calcium Phosphate Dibasic, 4 g Calcium Carbonate Light USP, 10 g corn oil, 3 g methionine, 2 g Choline Bitartrate, 1.5 g cholesterol NF, 1 g vitamin V10001C, 0.04 g ethoxyquin 17 40 43 5.0 4.67
Grain-based chows
Envigo, G18% Rodent chow Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, dicalcium phosphate, brewers yeast, iodized salt 24 18 58 3.5 3.1
Envigo, S-2335 M/R chow Ground wheat, ground corn, dehulled soybean meal, porcine fat preserved with BHA, dried whey casein, brewers dried yeast, porcine meat and bone meal, soybean hulls, calcium carbonate, iodized salt 20 29 51 2.7 3.5
Lab Diet, 5015 Mouse chow Whole wheat, dehulled soybean meal, ground corn, wheat germ, brewers dried yeast, porcine animal fat preserved with BHA and citric acid, condensed whey solubles, calcium carbonate, salt, dried whey protein concentrate, soybean oil, mono and diglycerides of edible fats 20 26 54 2.4 3.83
Lab Diet, 5001 Rodent chow Ground corn, dehulled soybean meal, dried beet pulp, fish meal, ground oats, brewers dried yeast, cane molasses, dehydrated alfalfa meal, dried whey, wheat germ, porcine animal fat preserved with BHA, porcine meat meal, wheat middlings, salt 29 13 58 5.2 3.36
Lab Diet, 5021 Autoclavable Mouse chow Ground corn, wheat middlings, dehulled soybean meal, wheat germ, fish meal, whole wheat, porcine animal fat preserved with BHA and citric acid, brewers dried yeast, soybean oil, ground oats, dried beet pulp, salt 23 24 53 3.7 3.72
Lab Diet, 5066 Rodent chow Ground corn, wheat middlings, dehulled soybean meal, animal fat preserved with BHA, fish meal, dehydrated alfalfa meal, cane molasses, calcium carbonate, salt, ground oats, ground wheat, ground soybean hulls, dried beet pulp, wheat germ, dried whey, dl-methionine, dicalcium phosphate, menadione dimethylpyrimidnol bisulfate, corn gluten meal 21 15 65 5.0 3.4
Custom-made diets
Custom-made low FODMAP purified ingredient diet (Envigo, Teklad Custom Diet TD.170455) Per 1000 g: 349.222 g corn starch, 200 g casein, 132 g maltodextrin, 100 g sucrose, 70 g lard, 50 g cellulose, 35 g mineral mix AIN-93G-MX 94046, 30 g soybean oil, 15 g vitamin mix AIN-93G-VX 94047, 4.75 g fructooligosaccharide, 3.56 g fructose, 3 g L-Cystine, 2.8 g calcium phosphate dibasic, 2.75 g choline bitartrate, 1.2 g galactooligosaccharide, 0.6 g sorbitol, 0.1 g green food color, 0.014 g antioxidant TBHQ, 0.002 g vitamin K phylloquinone, 0.002 g biotin 18 24 58 5.6 3.9
Custom-made high FODMAP purified ingredient diet (Envigo, Teklad Custom Diet TD.170456) Per 1000 g: 282.832 g corn starch, 200 g casein, 132 g maltodextrin, 100 g sucrose, 70 g lard, 50 g cellulose, 35 g mineral mix AIN-93G-MX 94046, 30 g soybean oil, 15 g vitamin mix AIN-93G-VX 94047, 35 g fructose, 24.0 g fructooligosaccharide, 11.5 g sorbitol, 6 g galactooligosaccharide, 3 g L-Cystine, 2.8 g calcium phosphate dibasic, 2.75 g choline bitartrate, 0.1 g green food color, 0.014 g antioxidant TBHQ, 0.002 g vitamin K phylloquinone, 0.002 g biotin 19 24 57 8.0 3.8