Fig. 5. Impact of reduced in vivo myocyte PKG activity on CHIP.

a Fluorescent confocal images of transverse myocardial sections with antibody staining for ubiquitin (green), cardiac troponin T (cTnT, red), and nuclei (DAPI, blue). There is increased myocyte ubiquitin staining after MI in mPDE5/oe myocardium. b Fluorescent confocal images of same myocardium stained for CHIP (green), cTnT, and DAPI. Myocyte CHIP expression is reciprocally reduced in mPDE5/oe heart after MI. Each examination performed with biological duplicates. c Representative Western blot and summary data for total CHIP expression in both experimental groups at baseline (CON) and after MI. n = 6 biological replicates, 2WANOVA, p = 0.0011 for interaction of group and +/− MI; Tukey mct: *p < 5 × 10⁻⁶. d Example immunoblot and summary results for phospho-S20 CHIP, and ratio of pCHIP to total CHIP in peri-infarct zone before and after MI. n = 6 biological replicates; 2WANOVA (p = 0.0002 for interaction term); Tukey mct: *p = 10⁻⁵. e Myocardial PKG activity directly correlates with levels of p/t CHIP. Data are from MI study with rest and post MI data pooled in the two groups. Source data are provided as a Source Data file. Individual data and mean ± SEM shown in each summary panel.