Fig. 6. PKG regulates CHIP-chaperone interaction and post-translational stability.

a Structural model of binding region between Hsc70 lid motif and the TPR region in CHIP. The location of S20 in reference to opposing lysine residues K597 and K601 in Hsc70 is shown. b Upper: example immunoprecipitation using Hsc70 as bait, and detecting Myc-tagged WT, SE, or SA CHIP in both control and SI conditions. There is minimal Hsc70-CHIP co-precipitation when CHIP-SA is expressed. Lower: summary data, n = 3 biological replicates/group, 1WANOVA for each condition, Holms-Sidaks mct: *p = 1.2 × 10⁻⁴, ^†0.003, ^‡0.0005, ^§0.02. c Immunoprecipitation gels and summary data for Hsc70 and CHIP interaction in vivo with and without MI in mice with mPDE5oe and littermate controls (CON). Hsc70 is bait, and antibody detection using CHIP and Hsc70. N = 4 biological replicates/group, Kruskal-Wallis test, Dunns mct. P values in figure. Source data are provided as a Source Data file. Individual data and mean ± SEM shown in each summary panel.