Figure 4.

Nox isoforms differentially regulate Ang II-induced ROS production in human VSMC. Cells were stimulated with Ang II (100 nmol/L) for 5 and 15 min in the presence and absence of NoxA1ds (Nox1 inhibitor, 10 μmol/L), GKT137831 (Nox1/Nox4 inhibitor, 10 μmol/L) and melittin (Nox5 inhibitor, 100 nmol/L). NADPH dependent O₂⁻ production (A) and H₂O₂ levels (B) were measured by lucigenin-derived chemiluminescence and amplex red, respectively. Results are expressed as mean ± SEM of 6–8 separate experiments. *P < 0.05 vs. Ctl, ⁺P < 0.05 vs. respective Ang II group. Ang II angiotensin II, Ctl control, H₂O₂ hydrogen peroxide, RLU relative light units.