Skip to main content
. 2020 Oct 20;10:17818. doi: 10.1038/s41598-020-73751-4

Figure 5.

Figure 5

Effect of lipid-raft disrupters on phosphorylation of MLC20, Ezrin/Radixin/Moesin, p53 and ERK1/2 in Ang II-stimulated human VSMCs. VSMCs were stimulated with Ang II (100 nmol/L) for 5 and 15 min in the presence and absence of MCD (10 mmol/L) or Nys (50 μg/mL). In some experiments, after MCD treatment, cholesterol was reloaded with cholesterol:MCD (1–10 mmol/L) complex. Phosphorylation of MLC20 (A), Ezrin–Radixin–Moesin (B), p53 (C) and ERK1/2 (D) were analysed by western blot. β-Actin was used as loading control. Bar graphs are means ± SEM from 5–7 experiments. Control was taken as 100% and data are presented as the percentage changes relative to control conditions. *P < 0.05 vs. Ctl. Ang II angiotensin II, Ctl control, MCD methyl-b-cyclodextrin, Nys nystatin, Chol cholesterol, p-MLC20 phosphorylated MLC20, p-ERM phosphorylated Ezrin–Radixin–Moesin, p-p53 phosphorylated p53, p-ERK1/2 phosphorylated ERK1/2.