Figure 7.

Effect of Nox5 silencing on ROS production and Ang II-induced signaling in hVSMC. Cells were transfected with Nox5 or control (Ctl) siRNA and stimulated with Ang II (100 nmol/L) for 5 min. NADPH-derived O₂⁻ generation was measured by lucigenin assay (A). H₂O₂ levels were measured by the Amplex Red assay (B). Representaive images of phospho-Ezrin–Radixin–Moesin, phospho-p53, phospho-ERK1/2 and Nox5 detected by western blot. α-tubulin and total ERK1/2 were used as loading control (C). Protein quantification of Ezrin–Radixin–Moesin (D), p53 (E) and ERK1/2 (F) phosphorylation in hVSMCs. Data are means ± SEM from 4–7 experiments. Control was taken as 100% and data are presented as the percentage changes relative to control conditions. *P < 0.05 vs. control, ⁺P < 0.05 vs. AngII Ctl siRNA. Ang II angiotensin II, Ctl control, H₂O₂ hydrogen peroxide, RLU relative light units, p-MLC20 phosphorylated MLC20, p-ERM phosphorylated Ezrin–Radixin–Moesin, p-p53 phosphorylated p53, p-ERK1/2 phosphorylated ERK1/2.