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. 2020 Oct 20;10:17726. doi: 10.1038/s41598-020-74839-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Enrollment of hypothermic oxygenated machine perfusion (HOPE) preserved allografts, compared to a historical cohort transplanted after conventional cold storage (CCS) at the University Hospital RWTH Aachen, Aachen, Germany. (B) Connecting the renal artery to the renal artery cannula following back-table preparation. (C) Positioning of the allograft in the kidney holder mesh of the device. (D) The kidney holder mesh is closed. After its placement into the organ container the allograft is ready to be perfused. (E) Hypothermic oxygenated machine perfusion of the kidney allograft with the Kidney Transport Assist (Organ Assist b.v., Groningen, The Netherlands). ECD, Extended criteria donor; DBD, Donation after brain death, KT, kidney transplantation.