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. 2020 Oct 20;10:17807. doi: 10.1038/s41598-020-74664-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Postnatal mammary gland development in mice with embryonal Sox10 deletion. (a–l) Carmine-alum and X-gal stainings were performed at P21 (a–c), P28 (d–f), P42 (g–i) and P56 (j–l) on Sox10^+/+ (wt in a,d,g,j) and Sox10^+/lacZ (b,c,e,f,h,i,k,l) mice. Inlays in right upper corner show magnifications of boxed areas in main panels. (m,n) The number of terminal end buds (TEB in m) and ductal tips (n) was quantified in mammary glands of Sox10^+/+ (grey hatched bars) and Sox10^+/lacZ (black hatched bars) mice at P21, P28, P42 and P56 (n = 4–7). Presentations are as absolute cell numbers ± SEM. Statistical significance between age-matched Sox10^+/+ and Sox10^+/lacZ was determined by Student`s t-test (***, P ≤ 0.001). Scale bar: 2 mm (a).