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. 2020 Oct 20;11:5300. doi: 10.1038/s41467-020-19150-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Scheme illustrating the genetic clonal tracing strategy using R26R-Confetti multicolor reporter mouse line crossed with tamoxifen-inducible Sma-CreERT2 mice. b Time course of 4-hydroxytamoxifen (4’OH-Tam) injections and analyses of independent unicolor clones represented on a time scale. Low doses of 4′OH induce low-frequency recombination. c 3D reconstructions of conductive and mixed clones induced at E7.75 and observed within Sma-CreERT2::R26R-Confetti opened-left ventricles at P21 of control (Ctrl) and Nkx2-5^+/− left ventricle. Whole-mount immunostaining for Contactin-2 (CNTN2) is used to distinguish conductive cells (CNTN2+) as indicate by arrowheads. d Conductive versus mixed clones frequency evolution upon time-course inductions quantified in P21 control (Ctrl) and Nkx2-5^+/− left ventricle. The progressive decrease of mixed clones frequency over time illustrates the kinetics of Sma+ cardiac progenitors segregation. e Diagram of the relative frequency of mixed clone sizes induced at E8.5 in P21 control and Nkx2-5^+/− left ventricles. P value is derived from non-parametric Mann–Whitney t-test, **p < 0.01.