Table 1.
Quantitative analysis of Cx40+-derived clones.
| 4’OH E9.5 | 4’OH E10.5 | 4’OH E14.5 | 4’OH E18.5 | |||||
|---|---|---|---|---|---|---|---|---|
| Ctrl | Nkx2-5+/− | Ctrl | Nkx2-5+/− | Ctrl | Nkx2-5+/− | Ctrl | Nkx2-5+/− | |
| Total hearts | 10 | 15 | 16 | 10 | 8 | 9 | 6 | 4 |
| Total clones | 36 | 48 | 64 | 50 | 118 | 128 | 105 | 58 |
| Frequency/heart | 3.6 | 3.2 | 4 | 5 | 14.75 | 14.2 | 17.5 | 14.5 |
| % GFP+ | 39.1 | 30.4 | 37.5 | 44 | 39.3 | 37.5 | 34.3 | 37.9 |
| % CFP+ | 4.3 | 4.3 | 1.6 | 2 | 0.85 | 0 | 0.95 | 0 |
| % RFP+ | 43.5 | 52.2 | 42.2 | 40 | 38.5 | 41.4 | 45.7 | 43.1 |
| % YFP+ | 13.0 | 13.0 | 18.75 | 14 | 21.4 | 21.1 | 19.0 | 19.0 |
| % Cond. clones | 36.1 | 47.9 | 53.1 | 18 | 57.6 | 32.8 | 95.2 | 44.8 |
| % Mixed clones | 27.8 | 25 | 12.5 | 16 | 11.0 | 9.4 | 0 | 17.2 |
| % Non-cond. clones | 36.1 | 27.1 | 34.4 | 66 | 37 | 57.8 | 5 | 36.2 |
| Size cond. clones | 5.4 | 5.1 | 4.5 | 3.9 | 2.6 | 2.5 | 3.18 | 1.9 |
| Size mixed clones | 27.7 | 13.3 | 31.1 | 9.25 | 6.5 | 7.25 | 0 | 6 |
| Size non-cond. clones | 16.9 | 27.7 | 31.6 | 16.1 | 6.5 | 6.7 | 4.8 | 3.0 |
Table represents the n values of hearts and clones analyzed in this study, using Cx40-CreERT2::R26R-Confetti mouse line. Only clones observed in the subendocardial surface of the inter-ventricular septum within the left ventricle are analyzed. Percentages of colors and type of clones are represented. The size of clones are measured by quantifying the number of cells per clone.