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. 2020 Oct 20;11(10):879. doi: 10.1038/s41419-020-03101-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A, B Downregulation of hTERT expression at protein and mRNA levels in H1299 and A549 cell lines treated with IPO13 siRNA for 48 h. C MTT assay used to detect cell viability in (H1299 and A549) cells transfected with IPO13 siRNA (n = 3, **P < 0.01, ***P < 0.001). D Cell proliferation assay in H1299 and A549 cells upon stable knockdown of IPO13 then overexpresses hTERT (n = 3, **P < 0.01, ***P < 0.001, ****P < 0.0001). E Pull-down assay conducted in the nuclear extract of H1299 and A549 cells to evaluate the binding of RFPL3 to hTERT promoter (−378 to +60). NSP, nonspecific. F The interaction between RFPL3 and hTERT promoter (−160 to +60) in H1299 cells was validated by ChIP assay. G A schematic model of the nuclear transport mechanism of RFPL3. Importin 13 mediates the nuclear transport of RFPL3 by recognizing an active NLS at the C terminal. RFPL3 nuclear translocation, in coordination with CBP’s transcriptional co-activation, promotes hTERT transcription. NPC nuclear pore complex, TF transcription factor, CBP CREB-binding protein.