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. 2020 Oct 20;11:5303. doi: 10.1038/s41467-020-18947-y

Fig. 2. AKAP5 is necessary for glucose/NF546-induced cAMP synthesis.

Fig. 2

Averaged ICUE3-PM FRET traces (mean = solid line; SEM = shade) and plot of maximum FRET ratio for wild-type (WT) and AKAP5−/− male arterial myocytes in response to a, b 20 mM D-glucose (D-glu; n = 21 cells/6 WT mice; n = 13 cells/6 AKAP5−/− mice), c, d 500 nM NF546 (n = 23 cells/6 WT mice; n = 18 cells/6 AKAP5−/− mice), and e, f 20 mM D-glu + NF546 (n = 22 cells/6 WT mice; n = 13 cells/6 AKAP5−/− mice) and 1 µM forskolin. *P < 0.05 with two-tailed Mann–Whitney test. The single asterisks highlight significant differences between datasets in the absence of forskolin. The double asterisks indicate a statistical difference within the same experimental group in the absence and presence forskolin. All significant P values are <0.0001. Data represent mean ± SEM. Source data are provided as Source data file.