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Quantitative correlation between mkDNA copy number and threshold cycle of the Leishmania qPCR assay. (A) Leishmania mkDNA plasmid was diluted in serial from 101 to 108 copies/reaction and subjected to qPCR. ΔRn = Rn (normalized reporter)—baseline. (B) Linear regression of Cq vs. lg copy number of mkDNA plasmid. Ct, Cycle threshold.
