Table 3.
Sensitivity and specificity of parasite detection and qPCR to diagnose cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL).
| Type | PCR Sample | Parasite+/qPCR+ | Parasite–/qPCR+ | Parasite+/qPCR– | Parasite–/qPCR– (Recovered) | Parasite/qPCR Sensitivity% | Specificity% | Parasite/qPCR Accuracy% |
|---|---|---|---|---|---|---|---|---|
| CL(n = 20) | Skin lesion | 20 | 0 | 0 | 0 | 100% (20/20) 100% (20/20) | NA | 100% (20/20) 100% (20/20) |
| VL (n = 30) | Bone marrow/PBMC | 20 | 2 | 1 | 7 | 91.3% (21/23) 95.6% (22/23) | NA | 93.3% (28/30) 96.6% (29/30) |
| Non-Leish diseases* (n = 11) | Bone marrow/PBMC | 0 | 0 | 0 | 11 | NA | ||
| Normal (n = 5) | Bone marrow/PBMC | 0 | 0 | 0 | 5 | NA | ||
| Total (n = 66) | Skin lesion/Bone marrow/PBMC | 40 | 2 | 1 | 23 | 95.3% (41/43)97.6% (42/43) | 100% | 96.9% (64/66) 98.5% (65/66) |
*
Including 5 with malaria, 5 with brucellosis, and 1 with dengue fever.