Table 2.

Experimental matrix for the fractional factorial design.

Experiment	Random order	SLM solvent	Buffer pH	Buffer volume (μl)	Stirring time (min)	Stirring rate (rpm)
1	14	Octanol	10.5	100	15	2,400
2	15	Octanol	9.5	500	15	2,400
3	9	Octanol	9.5	100	5	2,400
4	8	Octanol	10.5	500	15	1,200
5	5	Octanol	9.5	100	15	1,200
6	11	Decanol	9.5	500	5	2,400
7	1	Decanol	9.5	100	5	1,200
8	4	Decanol	10.5	500	5	1,200
9	6	Decanol	10.5	100	15	1,200
10	12	Octanol	10.5	500	5	2,400
11	16	Decanol	10.5	500	15	2,400
12	3	Octanol	9.5	500	5	1,200
13	10	Decanol	10.5	100	5	2,400
14	7	Decanol	9.5	500	15	1,200
15	13	Decanol	9.5	100	15	2,400
16	2	Octanol	10.5	100	5	1,200