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. 2020 Oct 7;14:582934. doi: 10.3389/fnins.2020.582934

FIGURE 4.

FIGURE 4

Increased immunoreactivity of Cx43 and Cx30 in areas around Aβ plaques in the RSP, MOp and MOs areas of cortical layer V and in the PO and VPM nucleus of the thalamus of 3 and 9-months-old 5XFAD mice. Double immunofluorescence staining of cortical and thalamic areas from 5XFAD and WT mice, with Aβ antibody (clone 6E10, green) and Cx43 or Cx30 (red). (A,B) 5XFAD mice at the age of 9-months showed increased immunoreactivity of Cx43 and Cx30 in the perimeter of Aβ plaques. Higher magnification images clearly show this phenomenon. (C–F) Quantification of the fluorescence intensity of Cx43 and Cx30 in areas around and away from Aβ plaques in 5XFAD mice and in areas in WT mice. Increased immunoreactivity of Cx43 and Cx30 was detected in the perimeter of Aβ plaques compared to areas off Aβ plaques in 5XFAD mice and in WT areas. The statistical analysis was performed by one-way ANOVA followed by Kruskal-Wallis multiple comparisons test [5XFAD mice/age (n = 6), WT mice/age (n = 6)]. Graphs show the mean and error bars indicate the standard error of the mean (SEM). Significance is given as: *p = 0.0332, **p = 0.0021, ***p = 0.0002, ****p < 0.0001. Scale bars = 50 μm in (A,B); 25 μm in higher magnification insets.