Fig. 8.

Hypertension reduced eNOS and induced iNOS expression in old mice. Western blot analysis of kidney tissue obtained from mice treated without or with Ang-II. (A) The bands are showing expression of eNOS and iNOS in an equal amount of loaded proteins. Loading was normalized with β-actin. (B) The bar diagrams indicate densitometric analysis of protein bands and are presented as mean fold change ± SD (n = 5). Significant difference *^{, †}p < 0.05 vs. WTY + Saline; ^‡p < 0.05 vs. other groups. (C) Immunostaining further confirmed that Ang-II downregulated eNOS (white arrows) and upregulated iNOS (yellow arrows) in aged mice. Representative images from eNOS and iNOS fluorescence stained kidney sections. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)