Fig. 9.

Hypertension downregulated Sirt1 and Sirt3 in the aged kidney. (A) Proteins were isolated from the kidney tissue and were analyzed by Western blotting. An equal amount of protein was loaded onto each well, electrophoresed, and blotted. The expression of each protein was normalized with β-actin. (B) Bands were quantified by densitometry, and the bar diagram represents fold change vs. WTY control. Data represents mean ± SD (n = 5). Significant difference *^{, †}p < 0.05 vs. WTY + Saline; ^‡p < 0.05 vs. other groups. (C) Representative images of Sirt1 and Sirt3 fluorescence staining in the kidney section. WTY mice demonstrated a high intensity of Sirt1 fluorescence in glomeruli (red) and Sirt3 in tubules (green). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)