Fig. 4.

Catalase knockdown in 3T3-L1 cells and MEFs promotes adipogenesis. (A) H₂O₂ concentration during early-stage differentiation of 3T3-L1 cells transfected with siCAT and CKO MEFs (n = 6). (B) Pref-1 and (C) GATA2 mRNA levels (n = 3). (D) Protein levels of catalase, C/EBPβ, and PPARγ relative to GAPDH in siCAT-transfected 3T3-L1 cells (n = 4). (E) Protein levels of catalase, C/EBPβ, and PPARγ relative to GAPDH in CKO MEFs (n = 4). (F) H₂O₂ concentration in MEFs from WT and CKO mice at D8 (n = 3). (G) ORO staining of MEFs from WT and CKO mice at D8 (n = 3). Data are expressed as the mean ± SEM; Student's t-test, *P < 0.05, **P < 0.01, ***P < 0.001 versus siCON-transfected 3T3-L1 cells or WT MEFs; ^tP < 0.05, ^ttP < 0.01, ^tttP < 0.001, siCON-transfected 3T3-L1 cells or WT MEFs at D0 versus siCON-transfected 3T3-L1 cells or WT MEFs at D2; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, siCON-transfected 3T3-L1 cells or WT MEFs at D0 versus siCAT-transfected 3T3-L1 cells or CKO MEFs at D2.