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Preoperative CTC fluorescence imaging in patients with HCC. The phenotype of CTCs was CEP8+/DAPI+/CD45- (DAPI: blue; CEP8: orange; CD45: red). When the CEP8 signal was ≥3, it was considered positive (A). When the CEP8 signal was ≤2, it was considered negative (B). The phenotype of leukocytes was CEP8+/CD45+/DAPI+ (C).
