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. 2020 Sep;8(18):1136. doi: 10.21037/atm-20-5115

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Ulinastatin ameliorates cerebral I/R injury. SD rats were divided into the sham, MCAO, and ulinastatin groups. Cerebral I/R injury was induced by MCAO. Ulinastatin was administered at a dosage of 300,000 U/kg 15 min after surgery. The brain tissues of the rats were collected. (A) HE and TUNEL staining of brain tissues observed, scale bar: 50 μm; (B) apoptosis rates of cells quantized with Image J (brown stained); (C) The brain tissues were stained with TCC. (D) The infraction volume characterized with TCC staining was quantized with Image J. (E) Relative protein expressions of caspase-3 and ICAM-1 were detected by Western blot and quantized by Image J. The bars show means ± SD of 4 independent experiments. **, P<0.01 compared with the sham group; ^##, P<0.01 compared with the MCAO group.