Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 20;11:5287. doi: 10.1038/s41467-020-19109-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Structural superposition of GnRH1R with active NTS1R (PDB ID: 6OS9, orange cartoon). Agonist JMV449 of NTS1R is shown magentas. Both elagolix and JMV449 bound to a shallow pocket that is located near the extracellular region of those two receptors. b The side chains of the hydrophobic Y^6.51-W^6.48-F^6.44 motif in TM6 are shown as sticks in GnRH1R (left), NTS1R (middle, PDB ID: 4S0V), and CRTH2R (right, PDB ID: 6D26), respectively. c Effects of GnRH agonist on activating GnRH1R WT, M125^3.36A, F272^6.40L, F272^6.40V, W280^6.48F, Y323^7.53F, and Y323^7.53A mutants expressed in HEK293T cells independently, monitored by IP1 accumulation assays. HEK293T were transiently transfected with the wild-type or mutant receptors (without PGS) and IP accumulation was measured after stimulation with GnRH ligands for 2 h. EC₅₀ values are expressed as means ± SEM (n = 3) at three times independently experiment repeats with similar results. d The rotamer comparison of NP^7.50xxY^7.53 motif of GnRH1R with inactive OX2R (PDB ID: 4S0V, green cartoon) and active NTS1R (PDB ID: 6OS9, orange cartoon) reveals an unusual conformation of Y^7.53 in GnRH1R. The side chain of F^1.53, W^1.60, and F^6.40 residues are shown as sticks with corresponding color to their receptors.