. 2020 Oct 20;10:17782. doi: 10.1038/s41598-020-74958-1

Table 3.

Effect of different additives on dry matter (g/kg FM), WSC (g/kg DM) and microbial counts (log₁₀ cfu/g FM) of alfalfa silage during ensiling.

Items	Treatment	Storage period				SEM	P-value
Items	Treatment	7	14	28	56	SEM	T	S	T × S
Dry matter	CK	211^cA	209^cAB	207^cAB	201^cB	0.109	0.004	0.043	0.335
	LP	221^b	218^b	217^b	214^b
	S	228^ab	225^ab	227^a	226^a
	LP + S	230^a	229^a	228^a	229^a
WSC	CK	16.1^bcA	14.5^abA	12.3^AB	9.73^B	0.051	0.022	0.009	0.054
	LP	13.1^cA	12.4^bAB	11.4^AB	8.83^B
	S	21.3^aA	17.2^aB	13.6^BC	11.2^C
	LP + S	17.5^abA	14.4^abAB	11.1^BC	8.80^C
LAB	CK	8.29^cA	8.16^AB	8.09^abAB	7.90^aB	0.195	0.003	0.006	0.019
	LP	8.63^bA	8.23^B	8.11^aBC	7.89^aC
	S	8.11^cA	8.02^AB	7.72^bcB	7.55^bB
	LP + S	8.94^aA	8.06^B	7.59^cC	7.36^bC
Clostridia	CK	2.13^aD	2.57^aC	3.08^aB	3.64^aA	0.019	0.016	0.020	0.038
	LP	1.72^bD	2.11^bC	2.45^bB	2.80^bA
	S	1.38^c	1.30^c	< 1.30	< 1.30
	LP + S	< 1.30	< 1.30	< 1.30	< 1.30
Enterobacteria	CK	7.22^aA	5.69^aB	4.91^aC	4.17^aD	0.033	0.011	0.009	0.022
	LP	5.03^bA	4.37^bB	3.64^bC	3.05^bD
	S	< 2.40	< 2.40	< 2.40	< 2.40
	LP + S	< 2.40	< 2.40	< 2.40	< 2.40

^a–d Means within a column with different superscripts differ (P < 0.05); ^A–EMeans within a raw with different superscripts differ (P < 0.05); LAB, lactic acid bacteria; LP, Lactobacillus plantarum; S, sucrose; LP + S, the addition of both sucrose and Lactobacillus plantarum; WSC, water soluble carbohydrates; DM, dry matter; FM, fresh matter; T: effect of treatment; S: effect of storage period; T × S: interaction between treatment and storage period; SEM, standard error of means.