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. 2020 Oct 20;10:17782. doi: 10.1038/s41598-020-74958-1

Table 5.

Alpha diversity of clostridial community in alfalfa silage during ensiling.

Treatments Days Reads OTUs Shannon Chao Coverage
CK 0 1279d 24ab 2.93ab 31a 0.99
7 7044cA 26a 3.40aA 33a 0.99
14 4035 cd 23ab 2.83bB 25abc 0.99
28 21154bA 18b 1.73cB 19bB 1.00
56 41069aA 5cC 0.09dC 7cC 0.99
LP 0 1266c 26a 3.04a 30a 0.99
7 3485cAB 23a 2.61abB 26a 1.00
14 4010bc 20ab 3.08aAB 22ab 0.99
28 7998bB 19ab 1.97bB 21abAB 0.99
56 28024aB 13bB 0.81cB 16bB 0.99
S 0 1187 25 2.96 32 0.99
7 4575AB 22 2.92B 29 0.99
14 2581 20 3.19AB 24 1.00
28 4099BC 22 3.04A 29A 0.99
56 3869C 19AB 3.22A 26A 0.99
LP + S 0 1243 24 2.97 32 0.99
7 2065B 23 2.94B 31 1.00
14 3855 22 3.23A 23 0.99
28 3394C 22 3.01A 26A 0.99
56 2300C 24A 3.17A 24AB 0.99
P-value T 0.030 0.035 0.027 0.029 0.162
S 0.019 0.047 0.006 0.037 0.540
T × S 0.051 0.111 0.102 0.035 0.771
SEM 669 0.911 0.716 1.13 0.0003

a–dMeans within a column with different superscripts differ (P < 0.05); A–EMeans within a raw with different superscripts differ (P < 0.05). CK, control; LP, Lactobacillus plantarum; S, sucrose; LP + S, the addition of both sucrose and Lactobacillus plantarum; T, effect of treatment; S, effect of storage period; T × S: interaction between treatment and storage period; SEM, standard error of means.