Table 5.
Alpha diversity of clostridial community in alfalfa silage during ensiling.
| Treatments | Days | Reads | OTUs | Shannon | Chao | Coverage |
|---|---|---|---|---|---|---|
| CK | 0 | 1279d | 24ab | 2.93ab | 31a | 0.99 |
| 7 | 7044cA | 26a | 3.40aA | 33a | 0.99 | |
| 14 | 4035 cd | 23ab | 2.83bB | 25abc | 0.99 | |
| 28 | 21154bA | 18b | 1.73cB | 19bB | 1.00 | |
| 56 | 41069aA | 5cC | 0.09dC | 7cC | 0.99 | |
| LP | 0 | 1266c | 26a | 3.04a | 30a | 0.99 |
| 7 | 3485cAB | 23a | 2.61abB | 26a | 1.00 | |
| 14 | 4010bc | 20ab | 3.08aAB | 22ab | 0.99 | |
| 28 | 7998bB | 19ab | 1.97bB | 21abAB | 0.99 | |
| 56 | 28024aB | 13bB | 0.81cB | 16bB | 0.99 | |
| S | 0 | 1187 | 25 | 2.96 | 32 | 0.99 |
| 7 | 4575AB | 22 | 2.92B | 29 | 0.99 | |
| 14 | 2581 | 20 | 3.19AB | 24 | 1.00 | |
| 28 | 4099BC | 22 | 3.04A | 29A | 0.99 | |
| 56 | 3869C | 19AB | 3.22A | 26A | 0.99 | |
| LP + S | 0 | 1243 | 24 | 2.97 | 32 | 0.99 |
| 7 | 2065B | 23 | 2.94B | 31 | 1.00 | |
| 14 | 3855 | 22 | 3.23A | 23 | 0.99 | |
| 28 | 3394C | 22 | 3.01A | 26A | 0.99 | |
| 56 | 2300C | 24A | 3.17A | 24AB | 0.99 | |
| P-value | T | 0.030 | 0.035 | 0.027 | 0.029 | 0.162 |
| S | 0.019 | 0.047 | 0.006 | 0.037 | 0.540 | |
| T × S | 0.051 | 0.111 | 0.102 | 0.035 | 0.771 | |
| SEM | 669 | 0.911 | 0.716 | 1.13 | 0.0003 |
a–dMeans within a column with different superscripts differ (P < 0.05); A–EMeans within a raw with different superscripts differ (P < 0.05). CK, control; LP, Lactobacillus plantarum; S, sucrose; LP + S, the addition of both sucrose and Lactobacillus plantarum; T, effect of treatment; S, effect of storage period; T × S: interaction between treatment and storage period; SEM, standard error of means.