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. 2020 Oct 20;10:17862. doi: 10.1038/s41598-020-74494-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MLH1 and STK11 SNV detection in paired ctDNA and tumor DNA samples using ddPCR. (a) Total number of positive droplet events in ddPCR analysis of the two genes. Gray bars represent the number of wild-type-positive droplets, and red bars represent the number of mutant-positive droplets. (b) Comparison of SNV detection results for the two genes between paired ctDNA and tumor DNA samples. Samples with positive, negative, and failed SNV detection are shown in blue, gray, and black, respectively. ctDNA circulating tumor DNA, ddPCR droplet digital polymerase chain reaction, SNV single-nucleotide variant.