Table 2.
Immunopositive DAPI signals in fields CA1 and CA3 of hippocampal sections from young and aged animals treated with control or IRβ AAVs.
| DAPI (mean gray value) | |||||||
|---|---|---|---|---|---|---|---|
| Young | Aged | p‐Values | |||||
| Control | IRβ | Control | IRβ | Age | AAV | ||
| CA1 | stratum pyramidale | 99.06 ± 16.32 | 153.07 ± 3.90 | 115.03 ± 14.29 | 149.08 ± 3.93 | n.s. | 0.005 |
| stratum oriens | 75.31 ± 12.65 | 113.89 ± 1.58 | 88.26 ± 11.79 | 116.83 ± 4.05 | n.s. | 0.007 | |
| stratum radiatum | 84.39 ± 13.66 | 129.82 ± 4.34 | 99.13 ± 11.77 | 132.78 ± 4.23 | n.s. | 0.003 | |
| CA3 | stratum pyramidale | 83.94 ± 13.45 | 127.16 ± 2.81 | 98.82 ± 12.29 | 131.63 ± 5.05 | n.s. | 0.005 |
A robust effect of IRβ treatment on field CA1 immunopositive DAPI signal across the three subfields measured was detected in both young and aged animals: stratum pyramidale (F (1,12) = 11.92, p = 0.005), stratum oriens (F (1,12) = 10.66, p = 0.007), and stratum radiatum (F (1,12) = 13.6, p = 0.003). Similarly, IRβ was also associated with increased DAPI signals in subfield stratum pyramidale of field CA3 (F (1,12) = 12.11, p = 0.005). No effect of age was noted in either of the fields measured (p > 0.05). Non‐significance is indicated by “n.s.” Data represent means ± SEM.