FIGURE 3.

SB225002 administration prevents atrial fibrosis, macrophages infiltration and superoxide production in SHRs. A, WKYs and SHRs at 2 mo of age were administered SB225002 intraperitoneally at a dose of 1 mg/kg/d for 4 mo. Collagen deposition in left atrial sections detected by Masson's trichrome staining (left). Quantification of fibrotic areas (right, n = 6). B, qPCR analysis of the mRNA levels of α‐SMA, collagen I and collagen III in the atria from WKYs and SHRs (n = 6). C, Immunohistochemical staining of macrophages with an anti‐Mac‐2 antibody (left). Quantification of the Mac‐2⁺ area (right, n = 6). D, DHE staining of atrial superoxide production (left). Quantification of DHE intensity (right, n = 6). Scale bar: 50 μm. Results are expressed as the mean ± SEM, and n represents the number of animals in each group