FIGURE 2.

Effect of shRNA‐RAGE and Ad‐RAGE on TG levels in mice. Old mice with HFD were injected with shRNA‐EGFP or shRNA‐RAGE virus, and RAGE knockdown efficiency was determined by real‐time PCR (b). In addition, assays for H&E and oil red O (ORO) staining of liver sections (a), Western blotting (b), and hepatic and serum TG levels (c) were performed. Middle‐aged wild‐type mice were injected with Ad‐GFP or Ad‐RAGE virus, and RAGE overexpression efficiency was determined by real‐time PCR (e). Assays for H&E‐ and oil red O‐stained liver sections (d), Western blotting (e), and hepatic and serum TG levels (f) were performed