FIGURE 3.

AGE and RAGE expression plays a role in hepatic fatty acid β‐oxidation. The expression levels of PPARα in mice of different ages were examined by Western blotting and real‐time PCR (a), and the expression levels of CPT1a, CPT1b, and MCAD were measured with real‐time PCR (b). (c) PPARα luciferase activity of the HEK293T cells transfected with Ad‐GFP or Ad‐RAGE. (d) PPARα efficiency was determined by real‐time PCR and Western blot analysis in the old HFD mice infected with shRNA‐EGFP or shRNA‐RAGE virus. (e) PPARα expression was determined by real‐time PCR and Western blot analysis in the middle‐aged WT mice infected with Ad‐GFP or Ad‐RAGE. (f–h) PPARα expression in the WT mice as determined by real‐time PCR and Western blot analysis (f), hepatic TG (g), and CPT1a, CPT1b, and MCAD efficiency as determined by real‐time PCR (h) in the primary hepatocytes transfected with Ad‐GFP or Ad‐RAGE