FIGURE 5.

Inhibition of IGF2BP1 attenuates cellular viability of TCam‐2/CDDP cells under CDDP treatment condition. IGF2BP1 was inhibited (IGF2BP1 siRNA) independently or together with METTL3 overexpression in TCam‐2/CDDP cells and cells were treated with CDDP (at the concentration of IC25) for 72 h. CCK8 cell viability assay was performed to determine cell viability (A). Apoptotic rate (B) was analysed by FCM assays. DNA damage rate was detected by gamma‐H2AX immunofluorescence assay (C). The data were presented as the mean ± SD of triplicate tests. *P < 0.05, ***P < 0.001, ns, not significant