FIGURE 8.

The effect of METTL3/IGF2BP1/TFAP2C on CDDP sensitivity of seminoma in vivo. Male mice were subcutaneously injected with tumour cells to establish xenografts (METTL3‐overexpressed TCam‐2/CDDP cells were inoculated at the initial time for once and IGF2BP1‐inhibited TCam‐2/CDDP cells were inoculated every 3 days). And at the same time, those mice were intraperitoneally injected with CDDP (6 mg/kg bodyweight) or vehicle every week for three doses. Then, the mice were humanely killed and their tumours were excised 25 days after inoculation. Tumour volumes were measured every third day for consecutive seven times (A). Representative photographs of IHC staining of Ki‐67 are presented (B). The protein expression levels of TFAP2C, WEE1 and BRCA1 were analysed by immunoblotting. GAPDH served as loading control (C). The data were presented as the mean ± SD of triplicate tests. IHC, immunohistochemistry. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant