FIGURE 2.

Selective inhibition of HDAC6 ameliorated renal injury in db/db mice. The activity of HDAC6 in the kidneys of mice treated with different stimuli (A), n = 5, 5 independent experiments. ACR was tested at the indicated time points(B), n = 6, 6 mice for each group. And the serum creatinine of mice treated with different stimuli (C), n = 5, 5 mice for each group. Representative immunohistochemical staining (D) and quantification (E) of WT1 in the glomerulus of mice at 21 wks old, n = 3, 3 mice for each group and 5 glomeruli for each mouse. Immunofluorescent staining (F) and the quantification of synaptopodin (G), n = 5, 5 mice for each group and 5 glomeruli for each mouse. ACR, albumin creatinine ratio; BKS, C57BL/KsJ; DMSO, dimethyl sulfoxide; HDAC6, histone deacetylase 6