Skip to main content
. 2020 Aug 28;24(19):11573–11582. doi: 10.1111/jcmm.15774

FIGURE 2.

FIGURE 2

Sensitivity of DLBCL lines to a IKKε/TBK1 inhibitor. A, Chemical structure of DMX3433. B and C, Dose‐response curves showing mean ± SEM cell survival (%) determined by ATP luminescence in response to treatment with DMX3433 for 48 h. IC50 (µmol/L) are shown at lower right. B, Dose‐response curves for Ly03, Ly10 and Pfeiffer (n = 3). C, Dose‐response curves for SUDHL4 (n = 1), SUDHL6 (n = 3), Toledo and Ly19 (n = 3). D and E, Nine IKKε/TBK1 inhibitors were assessed for effects on survival (determined by ATP luminescence) of the Ly10 and Ly03 cell lines. DMX3433 is indicated by the red dot. Inhibition of cell line growth (pEC50) (y‐axis) is plotted against the concentration required for 50% inhibition (pIC50) of the purified enzyme (x‐axis) (D) TBK1 enzyme activity or (E) IKKε activity. F and G, Time course (0, 0.5, 1, 2, 4 and 8 h) showing changes in, phosphorylated RELA (serine 536) and total RELA, phosphorylated STAT3 (tyrosine 705), total STAT3in response to DMX3433 (2 µmol/L) in (F) Ly10 and (G) Ly03. The numbers below the pRELA and pSTAT3 autorads are ratios of pRELA/GAPDH:RELA/GAPDH and pSTAT3/GAPDH:STAT3/GAPDH respectively. GAPDH is a loading control. Uncropped autorads are in the Appendix.