Figure 3.

Overexpression of ADAMTS9‐AS1 suppresses tumour growth in vitro and in vivo. A, qPCR analysis of ADAMTS9‐AS1 expression in Vector‐transfected and Lentivirus‐transfected CRC cells. B, xCELLigence system was used to monitor the cell growth dynamics of Vector‐transfected and Lentivirus‐transfected CRC cells. C, D, EdU staining assays were performed to detect the cell cycle. E, Colony formation assay was performed to determine the proliferation of CRC cells. F‐H, The stable ADAMTS9‐AS1‐overexpressing cell line, HT29, was used for in vivo assays. The tumours from two groups of nude mice are shown, and tumour growth curves were measured and shown after the injection. The tumour volume was calculated every 5 d. I, Ki67 protein levels in tumour tissues from oe‐ADAMTS9‐AS1 or negative control HT29 cells were determined by immunohistochemistry. The data represent the mean ± SD from three independent experiments. *P < .05, **P < .01