FIGURE 5.

Klotho overexpression affected the expression levels of Aβ transporters in aged APP/PS1 mice. (a) Representative immunofluorescence images of Aβ transporters (green) in the cortex (left) and choroid plexus (right), including low‐density lipoprotein receptor‐related protein 1 (LRP1), P‐glycoprotein (P‐gp), ATP‐binding cassette transporter A1 (ABCA1), and receptor for advanced glycation end products (RAGE). The lectin‐positive area (red) indicates micro‐vessels. Nuclei were stained with DAPI (blue). (b) Quantitative analysis of the levels of Aβ transporters in micro‐vessels and the choroid plexus. (c) Representative Western blotting and quantification of the relative protein levels of LRP1, P‐gp, ABCA1, and RAGE in brain tissue homogenates. Protein levels were normalized to GAPDH and are expressed as fold changes relative to the WT/LV‐GFP group. (d) Analyses of LRP1, P‐gp, ABCA1, and RAGE mRNA levels in brain tissue homogenates by qRT‐PCR. Relative mRNA levels were normalized to GAPDH and are expressed as fold changes relative to the WT/LV‐GFP group. (e) Soluble LRP1 (sLRP1) levels in serum, measured by ELISA. n = 6/group, except n = 4/group in (c). The data are expressed as mean ± SEM. The statistical analysis was performed using two‐way ANOVA followed by the Bonferroni‐Holm post hoc test. *p < 0.05, **p < 0.01, vs. APP/PS1/LV‐GFP group; ^# p < 0.05, ^## p < 0.01, vs. WT/LV‐GFP group