FIGURE 1.

Neuroblast and GMC divisions in the central brain during L3. (A) A schematic of the dorsal view of an L3 CNS, which is made up of the optic lobes (OL), the central brain (CB) and the ventral nerve cord (VNC) (Sousa-Nunes and Hirth, 2016). Type I neuroblasts (blue), which divide to self-renew and generate a GMC, are most abundant and are present throughout the CNS. GMCs divide symmetrically in size to generate two differentiating neuronal or glial progeny. Type II neuroblasts (magenta), defined by generation of two types of transit-amplifying progenitors (intermediate neural precursors and GMCs) consist of eight paired lineages found in the dorsoposterior regions of the CB. The black box indicates the region selected for live imaging, which contains only type I neuroblasts. (B) A time-series extracted from Movie 1 showing a cultured His2aV::eGFP brain (wL3) with a Type I neuroblast nucleus (yellow arrowhead) and a GMC nucleus (orange arrowhead) undergoing cell divisions. Timescale displayed as hh:mm:ss, scale bar = 10 μm.