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. 2020 Sep 3;9(9):824. doi: 10.3390/antiox9090824

Figure 2.

Figure 2

Effects of HD on TH, DAT and α-synuclein expression in SN of rotenone-treated mice. The immunohistochemical analysis has shown, compared with the Sham mice (A), a noticeable loss of TH-positive cells (B). Animals treated with HD revealed an increase in TH expression (C). *** p < 0.001 vs. Sham; *** p < 0.001 vs. rotenone (D). The immunohistochemical analysis revealed, compared with Sham group (E), an evident loss of DAT-positive cells (F). Animals subjected to treatment with HD revealed a positive stain for DAT (G) compared with rotenone group. *** p < 0.001 vs. Sham; ** p < 0.01 vs. rotenone (H). The midbrain was marked with antibodies against α–synuclein aggregation (I–K). The immunohistochemical analysis revealed, compared with Sham animals (I), a positive staining for α-synuclein (J). HD treatment appreciably reduced the positive staining for α-synuclein in the SN (K). *** p < 0.001 vs. Sham; *** p < 0.001 vs. rotenone (L). Data are expressed as the % of TH-positive pixels and are the means ± SEM of 5 mice/group. Scale bar: 20 μm.