Figure 3.

Effects of HD on α-synuclein expression and a double staining of TH/α–synuclein after rotenone-intoxication. Results are shown for Sham (A–C), mice after rotenone-intoxication (D–F) and mice treated with HD (G–I). Midbrain sections were double stained with antibodies against TH (B,E,H—green)/α-syn (A,D,G—red). Midbrain sections revealed an important expression of α-syn in dopaminergic neurons in the rotenone group (F), compared with Sham group (C). TH/α-syn, double stained, was decreased after HD treatment (I). The photographs are illustrative of at least three experiments performed on different days, and are representative of all animals in every group (N = 5 mice/group). Images were digitalized at a resolution of 8 bits into an array of 2048 × 2048 pixels. Scale bar: 100 μm.