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. 2020 Sep 3;9(9):824. doi: 10.3390/antiox9090824

Figure 4.

Figure 4

Effects of HD on Nrf2, HO-1, Hsp-70 and Sirt-1 proteins after rotenone intoxication. Western blot analysis demonstrated Nrf2 expression and Sirt-1 expression to be significantly increased in the rotenone group, whereas treatment with HD significantly further increased Nrf2 expression (A). HO-1 expression was increased after rotenone intoxication; treatment with HD decreased the levels of this protein (C). Hsp-70 expression was increased after rotenone intoxication; treatment with HD maintained high levels of this protein (D). Sirt-1 expression was increased after rotenone intoxication; treatment with HD decreased levels of this protein (E). Protein lysates were also incubated with a β-actin antibody (F) or laminin antibody (B) in order to verify that all samples had been loaded in equal quantities. The data are expressed as the mean ± SEM from N = 5 mice/group. (A) *** p < 0.001 vs. Sham; ** p < 0.01 vs. rotenone; (C) ** p < 0.01 vs. Sham; * p < 0.05 vs. rotenone; (D) ** p < 0.01 vs. rotenone; (E) * p < 0.05 vs. Sham; *** p < 0.001 vs. rotenone.