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. 2020 Sep 4;69(11):2467–2480. doi: 10.2337/db20-0585

Figure 5.

Figure 5

LRG1 modulates wound angiogenesis during wound healing. A: Representative immunofluorescence staining of CD31 (green) and DAPI (blue) (top) and quantification of vessel density (bottom) in day 7 wounds of wild-type and Lrg1−/− mice. Scale bar: 15 μm. B: Representative images of immunofluorescence staining detecting Ki67 (red) and DAPI (blue) (top) and quantification of percentage of Ki67+ cells (bottom) in HDMECs. Scale bar: 50 μm. C: Representative images (top) and quantification (bottom) of Matrigel tube formation. Scale bar: 125 μm. D: Representative images (top) and quantification (bottom) of Transwell migration assay. Scale bar: 100 μm. E: Representative Western blot (left) and densitometry analysis (right) of endothelial TGFβ-Smad1/5 signaling in HDMECs treated with rhLRG1 in the absence and presence of ALK1 inhibitor (LDN193189) or ALK5 inhibitor (SB431542). All images are representative, and data are represented as mean (95% CI; P) of n ≥ 6 mice or n ≥ 3 independent experiments per group. Significance was determined by unpaired, two-tailed Student t test. *P < 0.05, **P < 0.01, ***P < 0.001. WB, Western blot.