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. 2020 Oct 20;10:17768. doi: 10.1038/s41598-020-74746-x

Figure 2.

Figure 2

TMZ and irradiation treatment leads to a reduction in EGFR phosphorylation and expression in surviving cells. Cells with acquired resistance to temozolomide were assessed for active receptor tyrosine kinases using an RTK antibody arrays. Reduction in phospho-EGFR is shown in the red box. (A) U251R compared to U251MG cells (marked with a red box) and (B) graphically in U251R, U87R and U118R compared to their sensitive parental counterparts. (C) Parental and Resistant cells were probed for phospho and total levels of EGFR and GADPH expression by western blot and (D) assessed for EGFR gene expression by qPCR; *p < 0.05 relative to control. #20 and #41 cell were treated with TMZ (1 mM) and irradiation (5 Gy) for 7 days and then E. probed for phospho and total levels of EGFR and GADPH expression by western blot and (F) assessed for EGFR gene expression by qPCR; *p < 0.05 relative to control.