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. 2020 Oct 20;10:17768. doi: 10.1038/s41598-020-74746-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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#41R cells are more resistant to anti-EGFR inhibitors compared to control #41 cells. #41 (■) and #41R cells (□) were treated with ± 1 µM. (A) Erlotinib, (B) Gefitinib, (C) Afatinib or (D) Lapatinib for 72 h. Cell viability was then determined using a commercially available Cell Titer-Glo kit and samples read on a bioluminometer. Data is expressed as % viability compared to untreated control cells ± S.D.