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. 2020 Oct 7;11:569173. doi: 10.3389/fimmu.2020.569173

Figure 2.

Figure 2

miR-302/367 cluster regulates ROS production in macrophages. (A, B) MH-S cells were transfected with control or miR-302a and 302b mimics (A), control or miR-302a and 302b inhibitors (B). Twenty-four hours later, cells were infected with PAO1-GFP at MOI 10:1 for 1 h. Fluorescence intensity was calculated from triplicate samples (A, B). (C–H) MH-S cells were transiently transfected with control or miR-302a and miR-302b mimics (C–E), control or miR-302a and 302b inhibitors (F–H) for 24 h, and then either left uninfected or infected with PA14 for 1 h. Superoxide production in MH-S cells was detected by an NBT assay at a wavelength of 560 nm (C, F) and an H2DCF assay at a wavelength of 488 nm (D, G). Mitochondrial potential of MH-S cells was measured by JC-1 fluorescence assay at a wavelength of 532 nm (E, H). Data are shown as the mean ± SEM of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001. NS, not significant.